| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 31 | Acidithiobacillus ferrooxidans | CGCGAGGAAGTCAAAAGGC | TGCGCACCACTTCGATGA | 59.50 | 59.66 | 154 | 132 |
100.00%
|
100.00%
|
| Primer 32 | Acidithiobacillus ferrooxidans | GTTGTGCATTGGGTGACGG | AACATTGCCAAGCGCTGC | 59.71 | 60.05 | 299 | 130 |
100.00%
|
100.00%
|
| Primer 33 | Acidithiobacillus ferrooxidans | TTTTGGTGGCATCGCGTG | TCCACCAGGTCAGTCCGAT | 59.36 | 59.92 | 192 | 130 |
100.00%
|
100.00%
|
| Primer 34 | Acidithiobacillus ferrooxidans | CGGATCGCGAGGAAGTCAA | TGCGCACCACTTCGATGA | 59.86 | 59.66 | 159 | 130 |
100.00%
|
100.00%
|
| Primer 35 | Acidithiobacillus ferrooxidans | TTCCGGAGCGTGGATGAA | AAAGCGTTCGTCTGCGGA | 58.62 | 59.97 | 221 | 128 |
100.00%
|
100.00%
|
| Primer 36 | Acidithiobacillus ferrooxidans | TTTCCGGAGCGTGGATGAA | AAAGCGTTCGTCTGCGGA | 59.33 | 59.97 | 222 | 128 |
100.00%
|
100.00%
|
| Primer 37 | Acidithiobacillus ferrooxidans | GCTTGGCTGCACATTGGT | AACATTGCCAAGCGCTGC | 58.95 | 60.05 | 263 | 128 |
100.00%
|
100.00%
|
| Primer 38 | Acidithiobacillus ferrooxidans | TCGAGCGTGAGTTGATGCT | TGCGCACCACTTCGATGA | 59.41 | 59.66 | 201 | 128 |
100.00%
|
100.00%
|
| Primer 39 | Acidithiobacillus ferrooxidans | CAGACCTGCAATCCGGTGT | ATCAAGTTCCTGCCAGGCC | 60.00 | 60.00 | 284 | 127 |
100.00%
|
100.00%
|
| Primer 40 | Acidithiobacillus ferrooxidans | ACGCAATCCGGTCATGCA | GCCCAGATTGAGGATGGCA | 60.05 | 59.77 | 166 | 127 |
100.00%
|
100.00%
|